Phorbol ester effects on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured vascular smooth muscle cells.

Détails

ID Serval
serval:BIB_5FDA46C1CF7E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Phorbol ester effects on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured vascular smooth muscle cells.
Périodique
Life Sciences
Auteur(s)
Cotecchia S., Leeb-Lundberg L.M., Hagen P.O., Lefkowitz R.J., Caron M.G.
ISSN
0024-3205 (Print)
ISSN-L
0024-3205
Statut éditorial
Publié
Date de publication
1985
Volume
37
Numéro
25
Pages
2389-2398
Langue
anglais
Résumé
Tumor promoting phorbol esters stimulate Ca++ phospholipid-dependent protein kinase C. It has been suggested that this enzyme regulates the functional properties of different cell membrane receptors. In this study we investigated the effect of phorbol esters on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured smooth muscle cells derived from rabbit aorta. Treatment of these cells with biologically active phorbol esters for 15 min. to 2 hours caused a marked decrease of norepinephrine stimulation of inositol phospholipid metabolism and a 3 fold decrease in agonist affinity for 125I-HEAT binding to alpha 1-adrenoceptors in the intact smooth muscle cells. The ability of phorbol esters to modulate alpha 1-adrenoceptor responsiveness suggests that activation of protein kinase C may represent an important mechanism regulating alpha 1-adrenergic receptor functional properties.
Mots-clé
Animals, Aorta, Thoracic, Cells, Cultured, Muscle, Smooth, Vascular/drug effects, Muscle, Smooth, Vascular/metabolism, Norepinephrine/pharmacology, Phenethylamines/metabolism, Phorbol Esters/pharmacology, Phosphatidic Acids/metabolism, Phosphatidylinositols/metabolism, Prazosin/pharmacology, Rabbits, Receptors, Adrenergic, alpha/drug effects, Receptors, Adrenergic, alpha/metabolism, Tetradecanoylphorbol Acetate/pharmacology, Tetralones
Pubmed
Web of science
Création de la notice
24/01/2008 11:05
Dernière modification de la notice
20/08/2019 14:17
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