Immuno-affinity Amperometric Detection of Bacterial Infections.

Details

Serval ID
serval:BIB_5F940AA2AA46
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immuno-affinity Amperometric Detection of Bacterial Infections.
Journal
Angewandte Chemie
Author(s)
Zhu Y., Jović M., Lesch A., Tissières Lovey L., Prudent M., Pick H., Girault H.H.
ISSN
1521-3773 (Electronic)
ISSN-L
1433-7851
Publication state
Published
Issued date
05/11/2018
Peer-reviewed
Oui
Volume
57
Number
45
Pages
14942-14946
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
A combination of an immuno-affinity enrichment strategy and sensitive amperometric read-out was implemented in a point-of-care platform intended for bacterial infection analysis. Bacterial cells, selectively captured and enriched from complex matrices through immuno-affinity, were detected by amperometric monitoring of the redox state of metabolic activity indicators, providing species identification and viable-cell quantification. The method was successfully employed for the diagnosis of bacterial infections including antimicrobial susceptibility testing with only several hours of total working time.
Keywords
Antibodies, Immobilized/chemistry, Bacteria/isolation & purification, Bacterial Infections/diagnosis, Biosensing Techniques/instrumentation, Electrochemical Techniques/instrumentation, Equipment Design, Humans, Immunomagnetic Separation/instrumentation, Lab-On-A-Chip Devices, Point-of-Care Systems, bacterial infections, biosensors, electrochemistry, metabolic activity, point-of-care testing
Pubmed
Web of science
Create date
25/10/2018 11:30
Last modification date
24/09/2019 6:11
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