Body fluid and tissue analysis using filter paper sampling support prior to LC-MS/MS: application to fatal overdose with colchicine

Détails

ID Serval
serval:BIB_5F19B317CF7C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Body fluid and tissue analysis using filter paper sampling support prior to LC-MS/MS: application to fatal overdose with colchicine
Périodique
Drug Testing and Analysis
Auteur(s)
Lauer E., Widmer C., Versace F., Staub C., Mangin P., Sabatasso S., Augsburger M., Déglon J.
ISSN
1942-7611 (Electronic)
Statut éditorial
Publié
Date de publication
2013
Volume
5
Numéro
9-10
Pages
763-772
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Because of the various matrices available for forensic investigations, the development of versatile analytical approaches allowing the simultaneous determination of drugs is challenging. The aim of this work was to assess a liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform allowing the rapid quantification of colchicine in body fluids and tissues collected in the context of a fatal overdose. For this purpose, filter paper was used as a sampling support and was associated with an automated 96-well plate extraction performed by the LC autosampler itself. The developed method features a 7-min total run time including automated filter paper extraction (2 min) and chromatographic separation (5 min). The sample preparation was reduced to a minimum regardless of the matrix analyzed. This platform was fully validated for dried blood spots (DBS) in the toxic concentration range of colchicine. The DBS calibration curve was applied successfully to quantification in all other matrices (body fluids and tissues) except for bile, where an excessive matrix effect was found. The distribution of colchicine for a fatal overdose case was reported as follows: peripheral blood, 29 ng/ml; urine, 94 ng/ml; vitreous humour and cerebrospinal fluid, < 5 ng/ml; pericardial fluid, 14 ng/ml; brain, < 5 pg/mg; heart, 121 pg/mg; kidney, 245 pg/mg; and liver, 143 pg/mg. Although filter paper is usually employed for DBS, we report here the extension of this alternative sampling support to the analysis of other body fluids and tissues. The developed platform represents a rapid and versatile approach for drug determination in multiple forensic media.
Mots-clé
LC-MS/MS, colchicine, dried blood spots, intoxication, tissue analysis
Pubmed
Web of science
Création de la notice
24/01/2014 16:28
Dernière modification de la notice
03/03/2018 17:39
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