Intracellular nanomanipulation by a photonic-force microscope with real-time acquisition of a 3D stiffness matrix.

Détails

ID Serval
serval:BIB_5EF67A0BDBAE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Intracellular nanomanipulation by a photonic-force microscope with real-time acquisition of a 3D stiffness matrix.
Périodique
Nanotechnology
Auteur(s)
Bertseva E., Singh A.S., Lekki J., Thévenaz P., Lekka M., Jeney S., Gremaud G., Puttini S., Nowak W., Dietler G., Forró L., Unser M., Kulik A.J.
ISSN
1361-6528[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
20
Numéro
28
Pages
285709
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
A traditional photonic-force microscope (PFM) results in huge sets of data, which requires tedious numerical analysis. In this paper, we propose instead an analog signal processor to attain real-time capabilities while retaining the richness of the traditional PFM data. Our system is devoted to intracellular measurements and is fully interactive through the use of a haptic joystick. Using our specialized analog hardware along with a dedicated algorithm, we can extract the full 3D stiffness matrix of the optical trap in real time, including the off-diagonal cross-terms. Our system is also capable of simultaneously recording data for subsequent offline analysis. This allows us to check that a good correlation exists between the classical analysis of stiffness and our real-time measurements. We monitor the PFM beads using an optical microscope. The force-feedback mechanism of the haptic joystick helps us in interactively guiding the bead inside living cells and collecting information from its (possibly anisotropic) environment. The instantaneous stiffness measurements are also displayed in real time on a graphical user interface. The whole system has been built and is operational; here we present early results that confirm the consistency of the real-time measurements with offline computations.
Mots-clé
Lasers, Microscopy/instrumentation, Microscopy/methods
Pubmed
Web of science
Création de la notice
16/06/2010 11:19
Dernière modification de la notice
20/08/2019 14:16
Données d'usage