Canine distemper virus infection of primary hippocampal cells induces increase in extracellular glutamate and neurodegeneration.

Détails

ID Serval
serval:BIB_5EBBD58F029D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Canine distemper virus infection of primary hippocampal cells induces increase in extracellular glutamate and neurodegeneration.
Périodique
Journal of Neurochemistry
Auteur(s)
Brunner J.M., Plattet P., Majcherczyk P., Zurbriggen A., Wittek R., Hirling H.
ISSN
0022-3042[print], 0022-3042[linking]
Statut éditorial
Publié
Date de publication
2007
Volume
103
Numéro
3
Pages
1184-1195
Langue
anglais
Résumé
The canine distemper virus (CDV) belongs to the Morbillivirus genus which includes important human pathogens like the closely related measles virus. CDV infection can reach the nervous system where it causes serious malfunctions. Although this pathology is well described, the molecular events in brain infection are still poorly understood. Here we studied infection in vitro by CDV using a model of dissociated cell cultures from newborn rat hippocampus. We used a recombinant CDV closely related to the neurovirulent A75/17 which also expresses the enhanced green fluorescent protein. We found that infected neurons and astrocytes could be clearly detected, and that infection spreads only slowly to neighboring cells. Interestingly, this infection causes a massive cell death of neurons, which includes also non-infected neurons. Antagonists of NMDA-type or alpha-amino-3-hydroxy-5-methylisoxazole-4-propinate (AMPA)-type glutamate receptors could slow down this neuron loss, indicating an involvement of the glutamatergic system in the induction of cell death in infected and non-infected cells. Finally, we show that, following CDV infection, there is a steady increase in extracellular glutamate in infected cultures. These results indicate that CDV infection induces excitotoxic insults on neurons via glutamatergic signaling.
Mots-clé
Animals, Animals, Newborn, Astrocytes/metabolism, Astrocytes/virology, Cell Death/physiology, Cells, Cultured, Cercopithecus aethiops, Distemper/metabolism, Distemper/physiopathology, Distemper Virus, Canine/pathogenicity, Distemper Virus, Canine/physiology, Excitatory Amino Acid Antagonists/pharmacology, Extracellular Fluid/metabolism, Gliosis/metabolism, Gliosis/physiopathology, Glutamic Acid/metabolism, Green Fluorescent Proteins, Hippocampus/metabolism, Hippocampus/physiopathology, Nerve Degeneration/metabolism, Nerve Degeneration/physiopathology, Neurons/metabolism, Neurons/virology, Rats, Receptors, Glutamate/drug effects, Receptors, Glutamate/metabolism, Recombinant Proteins, Up-Regulation/physiology, Vero Cells
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 10:43
Dernière modification de la notice
20/08/2019 14:16
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