The outer limiting membrane (OLM) revisited: clinical implications.

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Serval ID
serval:BIB_5E67F90ABD0E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The outer limiting membrane (OLM) revisited: clinical implications.
Journal
Clinical Ophthalmology (auckland, N.z.)
Author(s)
Omri S., Omri B., Savoldelli M., Jonet L., Thillaye-Goldenberg B., Thuret G., Gain P., Jeanny J.C., Crisanti P., Behar-Cohen F.
ISSN
1177-5483 (Electronic)
ISSN-L
1177-5467
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
4
Pages
183-195
Language
english
Notes
Publication types: Journal ArticlePublication Status: epublish
Abstract
PURPOSE: The outer limiting membrane (OLM) is considered to play a role in maintaining the structure of the retina through mechanical strength. However, the observation of junction proteins located at the OLM and its barrier permeability properties may suggest that the OLM may be part of the retinal barrier.
MATERIAL AND METHODS: Normal and diabetic rat, monkey, and human retinas were used to analyze junction proteins at the OLM. Proteome analyses were performed using immunohistochemistry on sections and flat-mounted retinas and western blotting on protein extracts obtained from laser microdissection of the photoreceptor layers. Semi-thin and ultrastructure analyses were also reported.
RESULTS: In the rat retina, in the subapical region zonula occludens-1 (ZO-1), junction adhesion molecule (JAM), an atypical protein kinase C, is present and the OLM shows dense labeling of occludin, JAM, and ZO-1. The presence of occludin has been confirmed using western blot analysis of the microdissected OLM region. In diabetic rats, occludin expression is decreased and glial cells junctions are dissociated. In the monkey retina, occludin, JAM, and ZO-1 are also found in the OLM. Junction proteins have a specific distribution around cone photoreceptors and Müller glia. Ultrastructural analyses suggest that structures like tight junctions may exist between retinal glial Müller cells and photoreceptors.
CONCLUSIONS: In the OLM, heterotypic junctions contain proteins from both adherent and tight junctions. Their structure suggests that tight junctions may exist in the OLM. Occludin is present in the OLM of the rat and monkey retina and it is decreased in diabetes. The OLM should be considered as part of the retinal barrier that can be disrupted in pathological conditions contributing to fluid accumulation in the macula.
Pubmed
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23/08/2013 7:42
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20/08/2019 14:16
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