Analyzing the temporal regulation of translation efficiency in mouse liver.

Détails

Ressource 1Télécharger: BIB_5DC1F8E190CC.P001.pdf (580.82 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_5DC1F8E190CC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Analyzing the temporal regulation of translation efficiency in mouse liver.
Périodique
Genomics Data
Auteur(s)
Janich P., Arpat A.B., Castelo-Szekely V., Gatfield D.
ISSN
2213-5960 (Electronic)
ISSN-L
2213-5960
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
8
Pages
41-44
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Mammalian physiology and behavior follow daily rhythms that are orchestrated by endogenous timekeepers known as circadian clocks. Rhythms in transcription are considered the main mechanism to engender rhythmic gene expression, but important roles for posttranscriptional mechanisms have recently emerged as well (reviewed in Lim and Allada (2013) [1]). We have recently reported on the use of ribosome profiling (RPF-seq), a method based on the high-throughput sequencing of ribosome protected mRNA fragments, to explore the temporal regulation of translation efficiency (Janich et al., 2015 [2]). Through the comparison of around-the-clock RPF-seq and matching RNA-seq data we were able to identify 150 genes, involved in ribosome biogenesis, iron metabolism and other pathways, whose rhythmicity is generated entirely at the level of protein synthesis. The temporal transcriptome and translatome data sets from this study have been deposited in NCBI's Gene Expression Omnibus under the accession number GSE67305. Here we provide additional information on the experimental setup and on important optimization steps pertaining to the ribosome profiling technique in mouse liver and to data analysis.
Pubmed
Open Access
Oui
Création de la notice
29/03/2016 14:13
Dernière modification de la notice
20/08/2019 15:15
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