CRISPR-based gene expression control for synthetic gene circuits.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_5D99E1081188
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
CRISPR-based gene expression control for synthetic gene circuits.
Journal
Biochemical Society transactions
Author(s)
Santos-Moreno J., Schaerli Y.
ISSN
1470-8752 (Electronic)
ISSN-L
0300-5127
Publication state
Published
Issued date
30/10/2020
Peer-reviewed
Oui
Volume
48
Number
5
Pages
1979-1993
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Abstract
Synthetic gene circuits allow us to govern cell behavior in a programmable manner, which is central to almost any application aiming to harness engineered living cells for user-defined tasks. Transcription factors (TFs) constitute the 'classic' tool for synthetic circuit construction but some of their inherent constraints, such as insufficient modularity, orthogonality and programmability, limit progress in such forward-engineering endeavors. Here we review how CRISPR (clustered regularly interspaced short palindromic repeats) technology offers new and powerful possibilities for synthetic circuit design. CRISPR systems offer superior characteristics over TFs in many aspects relevant to a modular, predictable and standardized circuit design. Thus, the choice of CRISPR technology as a framework for synthetic circuit design constitutes a valid alternative to complement or replace TFs in synthetic circuits and promises the realization of more ambitious designs.
Keywords
Animals, Binding Sites, CRISPR-Cas Systems, Clustered Regularly Interspaced Short Palindromic Repeats, Gene Editing, Gene Expression, Gene Expression Profiling, Gene Expression Regulation, Gene Regulatory Networks, Genes, Synthetic, Genetic Engineering, Humans, Mice, Synthetic Biology, Transcription Factors/metabolism, CRISPR, gene expression and regulation, synthetic biological circuits, synthetic biology
Pubmed
Web of science
Open Access
Yes
Create date
26/09/2020 14:48
Last modification date
23/03/2023 7:11
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