Liver glucose transporter: a basolateral protein in hepatocytes and intestine and kidney cells.

Details

Serval ID
serval:BIB_5CC87DFE46D6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Liver glucose transporter: a basolateral protein in hepatocytes and intestine and kidney cells.
Journal
American Journal of Physiology.
Author(s)
Thorens B., Cheng Z.Q., Brown D., Lodish H.F.
ISSN
0363-6143
Publication state
Published
Issued date
12/1990
Volume
259
Number
6 Pt 1
Pages
C279-C285
Language
english
Abstract
The "liver" isoform of the facilitated diffusion glucose transporter is expressed predominantly in liver, intestine, kidney, and pancreatic islet beta-cells. The apparent molecular mass of the transporter in liver, kidney, and intestine is different, as detected by Western blot analysis of membrane proteins using antipeptide antibodies. However, as assessed by Northern blot analysis and molecular cloning, the same mRNA is expressed in these tissues, indicating that there are tissue-specific posttranslational modifications of the same transporter polypeptide. As determined by immunofluorescence analysis on frozen tissue sections, the liver glucose transporter is present on the sinusoidal membrane of hepatocytes, on the basolateral membrane of fully differentiated absorptive intestine epithelial cells, and on the basolateral membrane of proximal tubule cells of the kidney nephron. This localization is consistent with the involvement of the liver glucose transporter in several key steps of glucose metabolism: glucose uptake and release by the liver and absorption or reabsorption by epithelial cells of the intestine and kidney, respectively.
Keywords
Animals, Antibodies, Cell Membrane, Cells, Cultured, Fluorescent Antibody Technique, Gene Library, Intestines, Kidney, Liver, Monosaccharide Transport Proteins, RNA, RNA, Messenger, Rats
Pubmed
Create date
24/01/2008 13:41
Last modification date
20/08/2019 14:15
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