Generation of cytotoxic T lymphocytes in vitro. III. Velocity sedimentation studies of the differentiation and fate of effector cells in long-term mixed leukocyte cultures.
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State: Public
Version: Final published version
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Serval ID
serval:BIB_5CB97A3E5711
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Generation of cytotoxic T lymphocytes in vitro. III. Velocity sedimentation studies of the differentiation and fate of effector cells in long-term mixed leukocyte cultures.
Journal
The Journal of experimental medicine
ISSN
0022-1007
ISSN-L
0022-1007
Publication state
Published
Issued date
01/12/1974
Peer-reviewed
Oui
Volume
140
Number
6
Pages
1511-1521
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Separation of cells by velocity sedimentation at unit gravity was utilized to investigate the physical properties of cytotoxic thymus-derived lymphocytes (CTL) generated in long-term mixed leukocyte cultures (MLC). In kinetic studies, CTL were found almost exclusively in the large cell fractions at the peak of the response on day 4, whereas the majority of CTL in day 14 MLC had the sedimentation properties of small lymphocytes. Reculture until day 14 of cells fractionated on the basis of size on day 4 indicated that the small CTL were derived exclusively from cells which had been large on day 4. Re-exposure of day 14 MLC cells to the original stimulating alloantigens resulted in significant cell proliferation and rapid regeneration of CTL activity. Cell fractionation experiments demonstrated that the cells in the day 14 MLC population which responded to the secondary allogeneic stimulus were small T lymphocytes, and that these cells rapidly developed into large, highly cytotoxic CTL following stimulation. Moreover, by restimulating on day 14 fractions which were selected on the basis of size on day 4, it was found that the responding small lymphocytes were themselves the progeny of cells which were large at the peak of the response. Since CTL and CTL progenitors showed concomitant changes in physical properties with time, the possibility exists that they belong to the same cell lineage, and hence that CTL can differentiate into cells which are no longer cytotoxic, but capable of mounting an anamnestic response.
Keywords
Animals, Cell Differentiation, Cell Separation, Chromium Radioisotopes, Cytotoxicity Tests, Immunologic, Female, Histocompatibility Antigens, Immunity, Cellular, Kinetics, Lymphocyte Culture Test, Mixed, Mast-Cell Sarcoma/immunology, Mice, Mice, Inbred C57BL, Mice, Inbred DBA, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:13
Last modification date
09/08/2024 15:52