Peroxisomal and microsomal lipid pathways associated with resistance to hepatic steatosis and reduced pro-inflammatory state.

Détails

ID Serval
serval:BIB_5C7339688FC7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Peroxisomal and microsomal lipid pathways associated with resistance to hepatic steatosis and reduced pro-inflammatory state.
Périodique
Journal of Biological Chemistry
Auteur(s)
Hall D., Poussin C., Velagapudi V.R., Empsen C., Joffraud M., Beckmann J.S., Geerts A.E., Ravussin Y., Ibberson M., Oresic M., Thorens B.
ISSN
1083-351X[electronic], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
285
Numéro
40
Pages
31011-31023
Langue
anglais
Résumé
Accumulation of fat in the liver increases the risk to develop fibrosis and cirrhosis and is associated with development of the metabolic syndrome. Here, to identify genes or gene pathways that may underlie the genetic susceptibility to fat accumulation in liver, we studied A/J and C57Bl/6 mice that are resistant and sensitive to diet-induced hepatosteatosis and obesity, respectively. We performed comparative transcriptomic and lipidomic analysis of the livers of both strains of mice fed a high fat diet for 2, 10, and 30 days. We found that resistance to steatosis in A/J mice was associated with the following: (i) a coordinated up-regulation of 10 genes controlling peroxisome biogenesis and β-oxidation; (ii) an increased expression of the elongase Elovl5 and desaturases Fads1 and Fads2. In agreement with these observations, peroxisomal β-oxidation was increased in livers of A/J mice, and lipidomic analysis showed increased concentrations of long chain fatty acid-containing triglycerides, arachidonic acid-containing lysophosphatidylcholine, and 2-arachidonylglycerol, a cannabinoid receptor agonist. We found that the anti-inflammatory CB2 receptor was the main hepatic cannabinoid receptor, which was highly expressed in Kupffer cells. We further found that A/J mice had a lower pro-inflammatory state as determined by lower plasma levels and IL-1β and granulocyte-CSF and reduced hepatic expression of their mRNAs, which were found only in Kupffer cells. This suggests that increased 2-arachidonylglycerol production may limit Kupffer cell activity. Collectively, our data suggest that genetic variations in the expression of peroxisomal β-oxidation genes and of genes controlling the production of an anti-inflammatory lipid may underlie the differential susceptibility to diet-induced hepatic steatosis and pro-inflammatory state.
Mots-clé
partial least-squares, diet-induced obesity, high-fat diet, insulin-resistance, c57bl/6j mice, ischemia/reperfusion injury, metabolic syndrome, adipose-tissue, liver, expression
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/10/2010 7:45
Dernière modification de la notice
20/08/2019 14:14
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