Repression of flagellar genes in exponential phase by CsgD and CpxR, two crucial modulators of Escherichia coli biofilm formation.

Détails

ID Serval
serval:BIB_5C0EBC04840B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Repression of flagellar genes in exponential phase by CsgD and CpxR, two crucial modulators of Escherichia coli biofilm formation.
Périodique
Journal of Bacteriology
Auteur(s)
Dudin O., Geiselmann J., Ogasawara H., Ishihama A., Lacour S.
ISSN
1098-5530 (Electronic)
ISSN-L
0021-9193
Statut éditorial
Publié
Date de publication
2014
Volume
196
Numéro
3
Pages
707-715
Langue
anglais
Résumé
Escherichia coli adapts its lifestyle to the variations of environmental growth conditions, swapping between swimming motility or biofilm formation. The stationary-phase sigma factor RpoS is an important regulator of this switch, since it stimulates adhesion and represses flagellar biosynthesis. By measuring the dynamics of gene expression, we show that RpoS inhibits the transcription of the flagellar sigma factor, FliA, in exponential growth phase. RpoS also partially controls the expression of CsgD and CpxR, two transcription factors important for bacterial adhesion. We demonstrate that these two regulators repress the transcription of fliA, flgM, and tar and that this regulation is dependent on the growth medium. CsgD binds to the flgM and fliA promoters around their -10 promoter element, strongly suggesting direct repression. We show that CsgD and CpxR also affect the expression of other known modulators of cell motility. We propose an updated structure of the regulatory network controlling the choice between adhesion and motility.
Mots-clé
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Biofilms/growth & development, Cyclic AMP/genetics, Cyclic AMP/metabolism, Escherichia coli/physiology, Escherichia coli Proteins/genetics, Escherichia coli Proteins/metabolism, Flagella/genetics, Flagella/physiology, Gene Expression Regulation, Bacterial/physiology, Sigma Factor/genetics, Sigma Factor/metabolism, Trans-Activators/genetics, Trans-Activators/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/04/2014 16:50
Dernière modification de la notice
20/08/2019 15:14
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