Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy.

Détails

ID Serval
serval:BIB_5BFB4C9AC860
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy.
Périodique
Optics Express
Auteur(s)
Rappaz B., Marquet P., Cuche E., Emery Y., Depeursinge C., Magistretti P.
ISSN
1094-4087[electronic]
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
13
Numéro
23
Pages
9361-9373
Langue
anglais
Résumé
We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells in culture are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to lambda/1800, and the low acquisition time (~20micros) enable to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the integral refractive index and the cellular thickness (morphometry) from the measured phase shift is presented. Specifically, the method has been applied to study the dynamics of neurons in culture during a hypotonic stress. Such stress produces a paradoxical decrease of the phase which can be entirely resolved by applying the methodological approach described in this article; indeed the method allows to determine independently the thickness and the integral refractive index of cells.
Pubmed
Web of science
Création de la notice
10/03/2008 10:58
Dernière modification de la notice
20/08/2019 14:14
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