Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection.
Details
Serval ID
serval:BIB_5BF1C453B523
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection.
Journal
Polymers
ISSN
2073-4360 (Electronic)
ISSN-L
2073-4360
Publication state
Published
Issued date
08/06/2023
Peer-reviewed
Oui
Volume
15
Number
12
Pages
2607
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
We propose and demonstrate dendrimer-based coatings for a sensitive biochip surface that enhance the high-performance sorption of small molecules (i.e., biomolecules with low molecular weights) and the sensitivity of a label-free, real-time photonic crystal surface mode (PC SM) biosensor. Biomolecule sorption is detected by measuring changes in the parameters of optical modes on the surface of a photonic crystal (PC). We describe the step-by-step biochip fabrication process. Using oligonucleotides as small molecules and PC SM visualization in a microfluidic mode, we show that the PAMAM (poly-amidoamine)-modified chip's sorption efficiency is almost 14 times higher than that of the planar aminosilane layer and 5 times higher than the 3D epoxy-dextran matrix. The results obtained demonstrate a promising direction for further development of the dendrimer-based PC SM sensor method as an advanced label-free microfluidic tool for detecting biomolecule interactions. Current label-free methods for small biomolecule detection, such as surface plasmon resonance (SPR), have a detection limit down to pM. In this work, we achieved for a PC SM biosensor a Limit of Quantitation of up to 70 fM, which is comparable with the best label-using methods without their inherent disadvantages, such as changes in molecular activity caused by labeling.
Keywords
Pamam, Pc sm, biomolecular interaction, biosensor coating, dendrimer, hyperbranched polymers, label-free detection, microfluidics, oligonucleotides, photonic crystal surface mode biosensor, PAMAM, PC SM
Pubmed
Web of science
Open Access
Yes
Create date
29/06/2023 15:03
Last modification date
06/03/2024 8:16