Dopamine determines the vulnerability of striatal neurons to the N-terminal fragment of mutant huntingtin through the regulation of mitochondrial complex II.

Details

Serval ID
serval:BIB_5B73D0B91543
Type
Article: article from journal or magazin.
Collection
Publications
Title
Dopamine determines the vulnerability of striatal neurons to the N-terminal fragment of mutant huntingtin through the regulation of mitochondrial complex II.
Journal
Human Molecular Genetics
Author(s)
Benchoua A., Trioulier Y., Diguet E., Malgorn C., Gaillard M.C., Dufour N., Elalouf J.M., Krajewski S., Hantraye P., Déglon N., Brouillet E.
ISSN
1460-2083 (Electronic)
ISSN-L
0964-6906
Publication state
Published
Issued date
2008
Volume
17
Number
10
Pages
1446-1456
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
In neurodegenerative disorders associated with primary or secondary mitochondrial defects such as Huntington's disease (HD), cells of the striatum are particularly vulnerable to cell death, although the mechanisms by which this cell death is induced are unclear. Dopamine, found in high concentrations in the striatum, may play a role in striatal cell death. We show that in primary striatal cultures, dopamine increases the toxicity of an N-terminal fragment of mutated huntingtin (Htt-171-82Q). Mitochondrial complex II protein (mCII) levels are reduced in HD striatum, indicating that this protein may be important for dopamine-mediated striatal cell death. We found that dopamine enhances the toxicity of the selective mCII inhibitor, 3-nitropropionic acid. We also demonstrated that dopamine doses that are insufficient to produce cell loss regulate mCII expression at the mRNA, protein and catalytic activity level. We also show that dopamine-induced down-regulation of mCII levels can be blocked by several dopamine D2 receptor antagonists. Sustained overexpression of mCII subunits using lentiviral vectors abrogated the effects of dopamine, both by high dopamine concentrations alone and neuronal death induced by low dopamine concentrations together with Htt-171-82Q. This novel pathway links dopamine signaling and regulation of mCII activity and could play a key role in oxidative energy metabolism and explain the vulnerability of the striatum in neurodegenerative diseases.
Keywords
Animals, Cell Culture Techniques, Corpus Striatum/drug effects, Dopamine/pharmacology, Dopamine Agents/pharmacology, Down-Regulation/drug effects, Electron Transport Complex II/antagonists & inhibitors, Electron Transport Complex II/metabolism, Gene Expression/drug effects, Mutant Proteins/metabolism, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, Neurons/drug effects, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Protein Subunits/metabolism, Rats, Receptors, Dopamine D2/antagonists & inhibitors, Receptors, Dopamine D2/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
13/12/2011 16:18
Last modification date
20/08/2019 14:14
Usage data