The β-Ketoacyl-CoA Synthase HvKCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew.

Détails

ID Serval
serval:BIB_5AF6DBDCD081
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The β-Ketoacyl-CoA Synthase HvKCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew.
Périodique
Plant & Cell Physiology
Auteur(s)
Li C., Haslam T.M., Krüger A., Schneider L.M., Mishina K., Samuels L., Yang H., Kunst L., Schaffrath U., Nawrath C., Chen G., Komatsuda T., von Wettstein-Knowles P.
ISSN
1471-9053 (Electronic)
ISSN-L
0032-0781
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
59
Numéro
4
Pages
806-822
Langue
anglais
Résumé
The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH was deduced from wax and cutin analyses and by heterologous expression in yeast. Combined, these experiments revealed that CER-ZH/HvKCS1 has a substrate specificity for C16-C20, especially unsaturated, acyl chains, thus playing a major role in total acyl chain elongation for wax biosynthesis. The contribution of CER-ZH to water barrier properties of the cuticle and its influence on the germination of barley powdery mildew fungus were also assessed.

Mots-clé
Blumeria graminis f. sp. Hordei, Hordeum vulgare, cuticular wax, very-long-chain fatty acids, β-ketoacyl-CoA synthase
Pubmed
Web of science
Création de la notice
08/02/2018 18:13
Dernière modification de la notice
20/08/2019 14:13
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