Article: article from journal or magazin.
Apoptosis stimulated by the 91-kDa caspase cleavage MEKK1 fragment requires translocation to soluble cellular compartments.
Journal of Biological Chemistry
MEKK1, a 196-kDa mitogen-activated protein kinase (MAPK) kinase kinase, generates anti-apoptotic signaling as a full-length protein but induces apoptosis when cleaved by caspases. Here, we show that caspase-dependent cleavage of MEKK1 relocalizes the protease-generated 91-kDa kinase fragment from a particulate fraction to a soluble cytoplasmic fraction. Relocalization of MEKK1 catalytic activity is necessary for the pro-apoptotic function of MEKK1. The addition of a membrane-targeting signal to the 91-kDa fragment inhibits caspase activation and the induction of apoptosis but does not change the activation of JNK, ERK, NFkappaB, or p300. These results identify the caspase cleavage of MEKK1 as a dynamic regulatory mechanism that alters the subcellular distribution of MEKK1, changing its function to pro-apoptotic signaling, which does not depend on the currently described MEKK1 effectors.
Animals, Apoptosis, Blotting, Western, Catalysis, Cell Line, Cell Membrane/enzymology, Cell Nucleus/enzymology, Cytoplasm/enzymology, Cytoplasm/metabolism, DNA, Complementary/metabolism, Dose-Response Relationship, Drug, E1A-Associated p300 Protein, Humans, MAP Kinase Kinase Kinase 1, Mice, Microscopy, Fluorescence, Mitogen-Activated Protein Kinase 8, Mitogen-Activated Protein Kinases/metabolism, NF-kappa B/metabolism, Nuclear Proteins/metabolism, Plasmids/metabolism, Protein Binding, Protein Structure, Tertiary, Protein Transport, Protein-Serine-Threonine Kinases/chemistry, Protein-Serine-Threonine Kinases/metabolism, Subcellular Fractions/metabolism, Time Factors, Trans-Activators/metabolism, Transfection, Ultraviolet Rays
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