The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript.

Détails

ID Serval
serval:BIB_597DB80469A3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript.
Périodique
Genes & development
Auteur(s)
Malone C.D., Mestdagh C., Akhtar J., Kreim N., Deinhard P., Sachidanandam R., Treisman J., Roignant J.Y.
ISSN
1549-5477 (Electronic)
ISSN-L
0890-9369
Statut éditorial
Publié
Date de publication
15/08/2014
Peer-reviewed
Oui
Volume
28
Numéro
16
Pages
1786-1799
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Résumé
The exon junction complex (EJC) is a highly conserved ribonucleoprotein complex that binds RNAs during splicing and remains associated with them following export to the cytoplasm. While the role of this complex in mRNA localization, translation, and degradation has been well characterized, its mechanism of action in splicing a subset of Drosophila and human transcripts remains to be elucidated. Here, we describe a novel function for the EJC and its splicing subunit, RnpS1, in preventing transposon accumulation in both Drosophila germline and surrounding somatic follicle cells. This function is mediated specifically through the control of piwi transcript splicing, where, in the absence of RnpS1, the fourth intron of piwi is retained. This intron contains a weak polypyrimidine tract that is sufficient to confer dependence on RnpS1. Finally, we demonstrate that RnpS1-dependent removal of this intron requires splicing of the flanking introns, suggesting a model in which the EJC facilitates the splicing of weak introns following its initial deposition at adjacent exon junctions. These data demonstrate a novel role for the EJC in regulating piwi intron excision and provide a mechanism for its function during splicing.
Mots-clé
Animals, Argonaute Proteins/genetics, Argonaute Proteins/metabolism, DNA Transposable Elements/physiology, DNA, Complementary/metabolism, Drosophila/genetics, Drosophila/metabolism, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Female, Gene Knockdown Techniques, Gene Silencing, Introns/genetics, Mutation, Ovary/cytology, Ovary/metabolism, Protein Subunits/metabolism, RNA Splicing, Ribonucleoproteins/genetics, Ribonucleoproteins/metabolism, AGO3, EJC, Piwi, piRNA, splicing, transposon
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/10/2019 12:57
Dernière modification de la notice
29/10/2019 6:26
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