Agonist-induced beta-adrenergic receptor internalization on intact human mononuclear leukocytes: effect of temperature of mononuclear leukocyte separation.

Détails

ID Serval
serval:BIB_595E2A72C082
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Agonist-induced beta-adrenergic receptor internalization on intact human mononuclear leukocytes: effect of temperature of mononuclear leukocyte separation.
Périodique
Journal of Laboratory and Clinical Medicine
Auteur(s)
De Blasi A., Cotecchia S., Fratelli M., Lipartiti M.
ISSN
0022-2143 (Print)
ISSN-L
0022-2143
Statut éditorial
Publié
Date de publication
1986
Volume
107
Numéro
1
Pages
86-94
Langue
anglais
Résumé
The hydrophilic ligand 3H-CGP 12177 was used to measure beta-adrenergic receptors on intact human mononuclear leukocytes (MNLs). A single homogeneous class of receptor sites was found, with KD value of 0.71 +/- 0.04 nmol/L and Bmax of 3.0 +/- 0.4 fmol/10(6) cells (mean +/- SEM; n = 12). The receptor affinity (KD) and density (Bmax) were similar when measured on MNLs, purified lymphocytes, and a T-lymphocyte-enriched population from the same individual. Preincubation of intact MNLs with 1 mumol/L isoproterenol at 37 degrees C for 20 minutes reduced the number of surface receptors, measured by 3H-CGP 12177 binding at 4 degrees C for 20 hours, by approximately 70% (receptor internalization) without affecting KD. This effect was reversible, and surface receptors completely reappeared when binding was investigated at 37 degrees C for 40 minutes. Receptor internalization was similar when either isolated MNLs or whole blood was incubated with isoproterenol. Agonist-induced receptor internalization was stable during MNL isolation from whole blood at 4 degrees C but was partially or completely lost from MNLs prepared at 20 degrees C.
Mots-clé
Adrenergic beta-Agonists/pharmacology, Cell Separation, Humans, Isoproterenol/pharmacology, Leukocytes/analysis, Male, Propanolamines/metabolism, Receptors, Adrenergic, beta/analysis, Temperature, Tritium/diagnostic use
Pubmed
Web of science
Création de la notice
24/01/2008 11:06
Dernière modification de la notice
20/08/2019 14:12
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