A role for beta-actin in RNA polymerase III transcription.

Détails

ID Serval
serval:BIB_58FD29B86B98
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A role for beta-actin in RNA polymerase III transcription.
Périodique
Genes and Development
Auteur(s)
Hu P., Wu S., Hernandez N.
ISSN
0890-9369[print], 0890-9369[linking]
Statut éditorial
Publié
Date de publication
2004
Volume
18
Numéro
24
Pages
3010-3015
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
When transcription from the human U6 snRNA gene is reconstituted with recombinant factors and purified RNA polymerase III (pol III), pol III must be treated with CK2 to be active. We show that highly purified pol III contains associated beta-actin, and beta-actin localizes to an active U6 promoter in vivo. Pol III immunoprecipitated from IMR90 cells treated with a genotoxic agent lacks associated beta-actin and is inactive in the reconstituted assay. Transcription is regained upon treatment of pol III with CK2 and addition of beta-actin. This suggests that beta-actin associated with pol III is essential for basal pol III transcription.
Mots-clé
Actins/metabolism, Casein Kinase II/metabolism, Cells, Cultured, Humans, Immunoprecipitation, Microscopy, Fluorescence, Promoter Regions, Genetic/genetics, RNA Polymerase III/genetics, RNA Polymerase III/metabolism, RNA, Small Nuclear/genetics, Transcription, Genetic/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 15:12
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