Immobilization of proteinases on chitosan

Détails

Ressource 1Demande d'une copie Sous embargo indéterminé.
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_588DDDB20CEB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Immobilization of proteinases on chitosan
Périodique
Biotechnology Letters
Auteur(s)
Leuba J.L., Widmer F.
ISSN
0141-5492
Statut éditorial
Publié
Date de publication
1979
Peer-reviewed
Oui
Volume
1
Numéro
3
Pages
109-114
Langue
anglais
Résumé
Trypsin, pronase and subtilisin were immobilized on chitosan by glutaraldehyde coupling. Significant retention of activity was observed when synthetic substrates as well as casein were used. The specific activities of the bound proteinases ranged from 38% to 79% of their initial specific activities. The pH-activity profile of trypsin was slightly shifted toward alkaline values, and its thermal stability was increased. Immobilized trypsin was found to be less sensitive to its natural inhibitors than the soluble enzyme.
Web of science
Création de la notice
13/08/2015 7:53
Dernière modification de la notice
20/08/2019 14:12
Données d'usage