Fast and reliable PCR/sequencing/RFLP assay for identification of fungi in onychomycoses

Détails

ID Serval
serval:BIB_58372FD982B4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Fast and reliable PCR/sequencing/RFLP assay for identification of fungi in onychomycoses
Périodique
Journal of Medical Microbiology
Auteur(s)
Monod  M., Bontems  O., Zaugg  C., Lechenne  B., Fratti  M., Panizzon  R.
ISSN
0022-2615 (Print)
Statut éditorial
Publié
Date de publication
09/2006
Volume
55
Numéro
Pt 9
Pages
1211-6
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
Fusarium spp. and other non-dermatophyte fungi are repeatedly isolated from abnormal nails. To investigate whether these fungi are the aetiological agents of infection or simply transient contaminants, a PCR/sequencing/RFLP assay was developed for direct and routine identification of the infecting fungi in onychomycosis. Fungal DNA was readily extracted using a commercial kit after dissolving nail fragments in a Na2S solution. Amplification of part of the 28S rDNA by PCR was performed with universal primers and the fungal species were identified by sequencing. The PCR/sequencing results were comparable with microbiological identification from the same nail sample. In addition to dermatophytes, Fusarium spp. and other less frequently isolated non-dermatophyte fungi were identified as single fungal agents in onychomycosis. Moreover, mixed infections were clearly demonstrated in 10% of cases by RFLP analysis of PCR products. Identification of infectious agents could be obtained in 2 days, whilst results from fungal cultures take 1-3 weeks. Rapid and reliable molecular identification of the infectious fungus expedites the choice of appropriate antifungal therapy, thereby improving the cure rate of onychomycosis.
Mots-clé
DNA, Fungal/*genetics DNA, Ribosomal/genetics Fungi/*classification/genetics/*isolation & purification Humans Nails/microbiology Onychomycosis/*microbiology *Polymerase Chain Reaction *Polymorphism, Restriction Fragment Length RNA, Ribosomal, 28S/genetics Reproducibility of Results Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:54
Dernière modification de la notice
20/08/2019 14:12
Données d'usage