Article: article from journal or magazin.
Inducible gene targeting in mice.
A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.
Animals, Crosses, Genetic, DNA Nucleotidyltransferases/genetics, DNA Polymerase I/genetics, Female, GTP-Binding Proteins, Gene Targeting/methods, Genetic Vectors, Integrases, Interferon-alpha/pharmacology, Interferon-beta/pharmacology, Liver/drug effects, Liver/metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Poly I-C/pharmacology, Promoter Regions, Genetic, Proteins/genetics, Recombination, Genetic, Sequence Deletion, Viral Proteins
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