Neurochemical profile of the mouse hypothalamus using in vivo 1H MRS at 14.1T.

Détails

ID Serval
serval:BIB_57A93519A4EC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Neurochemical profile of the mouse hypothalamus using in vivo 1H MRS at 14.1T.
Périodique
NMR in Biomedicine
Auteur(s)
Lei H., Poitry-Yamate C., Preitner F., Thorens B., Gruetter R.
ISSN
1099-1492[electronic], 0952-3480[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
23
Numéro
6
Pages
578-583
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The hypothalamus plays an essential role in the central nervous system of mammals by among others regulating glucose homeostasis, food intake, temperature, and to some extent blood pressure. Assessments of hypothalamic metabolism using, e.g. (1)H MRS in mouse models can provide important insights into its function. To date, direct in vivo (1)H MRS measurements of hypothalamus have not been reported. Here, we report that in vivo single voxel measurements of mouse hypothalamus are feasible using (1)H MRS at 14.1T. Localized (1)H MR spectra from hypothalamus were obtained unilaterally (2-2.2 microL, VOI) and bilaterally (4-4.4 microL) with a quality comparable to that of hippocampus (3-3.5 microL). Using LCModel, a neurochemical profile consisting of 21 metabolites was quantified for both hypothalamus and hippocampus with most of the Cramér-Rao lower bounds within 20%. Relative to the hippocampus, the hypothalamus was characterized by high gamma-aminobutryric acid and myo-inositol, and low taurine concentrations. When studying transgenic mice with no glucose transporter isoform 8 expressed, small metabolic changes were observed, yet glucose homeostasis was well maintained. We conclude that a specific neurochemical profile of mouse hypothalamus can be measured by (1)H MRS which will allow identifying and following metabolic alterations longitudinally in the hypothalamus of genetic modified models.
Mots-clé
Animals, Glucose Transport Proteins, Facilitative/genetics, Glucose Transport Proteins, Facilitative/metabolism, Hypothalamus/anatomy & histology, Hypothalamus/chemistry, Magnetic Resonance Spectroscopy/methods, Male, Mice, Mice, Inbred C57BL, Mice, Knockout
Pubmed
Web of science
Création de la notice
04/08/2010 15:28
Dernière modification de la notice
20/08/2019 14:11
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