Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter.

Détails

Ressource 1Télécharger: s41467-017-01930-5.pdf (2266.11 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_578DBAD79CB3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter.
Périodique
Nature communications
Auteur(s)
Klompmaker S.H., Kohl K., Fasel N., Mayer A.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Statut éditorial
Publié
Date de publication
01/12/2017
Peer-reviewed
Oui
Volume
8
Numéro
1
Pages
1879
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Cells acquire free metals through plasma membrane transporters. But, in natural settings, sequestering agents often render metals inaccessible to transporters, limiting metal bioavailability. Here we identify a pathway for metal acquisition, allowing cells to cope with this situation. Under limited bioavailability of Mg <sup>2+</sup> , yeast cells upregulate fluid-phase endocytosis and transfer solutes from the environment into their vacuole, an acidocalcisome-like compartment loaded with highly concentrated polyphosphate. We propose that this anionic inorganic polymer, which is an avid chelator of Mg <sup>2+</sup> , serves as an immobilized cation filter that accumulates Mg <sup>2+</sup> inside these organelles. It thus allows the vacuolar exporter Mnr2 to efficiently transfer Mg <sup>2+</sup> into the cytosol. Leishmania parasites also employ acidocalcisomal polyphosphate to multiply in their Mg <sup>2+</sup> -limited habitat, the phagolysosomes of inflammatory macrophages. This suggests that the pathway for metal uptake via endocytosis, acidocalcisomal polyphosphates and export into the cytosol, which we term EAPEC, is conserved.
Mots-clé
Biological Transport, Cytosol/metabolism, Endocytosis, Leishmania/genetics, Leishmania/metabolism, Magnesium/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Vacuoles/genetics, Vacuoles/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/12/2017 21:28
Dernière modification de la notice
20/08/2019 14:11
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