Repairing a deleterious domestication variant in a floral regulator gene of tomato by base editing.
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State: Public
Version: Author's accepted manuscript
License: CC BY-NC-ND 4.0
UNIL restricted access
State: Public
Version: Author's accepted manuscript
License: CC BY-NC-ND 4.0
Secondary document(s)
Under embargo until 17/07/2025.
UNIL restricted access
State: Public
Version: Supplementary document
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UNIL restricted access
State: Public
Version: Supplementary document
License: Not specified
Serval ID
serval:BIB_5755A1C0CE68
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Repairing a deleterious domestication variant in a floral regulator gene of tomato by base editing.
Journal
Nature genetics
ISSN
1546-1718 (Electronic)
ISSN-L
1061-4036
Publication state
Published
Issued date
01/2025
Peer-reviewed
Oui
Volume
57
Number
1
Pages
231-241
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Crop genomes accumulate deleterious mutations-a phenomenon known as the cost of domestication. Precision genome editing has been proposed to eliminate such potentially harmful mutations; however, experimental demonstration is lacking. Here we identified a deleterious mutation in the tomato transcription factor SUPPRESSOR OF SP2 (SSP2), which became prevalent in the domesticated germplasm and diminished DNA binding to genome-wide targets. We found that the action of SSP2 is partially redundant with that of its paralog SSP in regulating shoot and inflorescence architecture. However, redundancy was compromised during tomato domestication and lost completely in the closely related species Physalis grisea, in which a single ortholog regulates shoot branching. We applied base editing to directly repair the deleterious mutation in cultivated tomato and obtained plants with compact growth that provide an early fruit yield. Our work shows how deleterious variants have sensitized modern genotypes for phenotypic tuning and illustrates how repairing deleterious mutations with genome editing may allow predictable crop improvement.
Keywords
Solanum lycopersicum/genetics, Gene Editing/methods, Domestication, Plant Proteins/genetics, Mutation, Gene Expression Regulation, Plant, Flowers/genetics, Flowers/growth & development, Genome, Plant, Phenotype, Transcription Factors/genetics, Crops, Agricultural/genetics
Pubmed
Web of science
Create date
08/01/2025 15:40
Last modification date
18/01/2025 7:13