Article: article from journal or magazin.
Novel markers of the human follicle-associated epithelium identified by genomic profiling and microdissection.
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
BACKGROUND & AIMS: Regulation of gene expression in the follicle-associated epithelium (FAE) over Peyer's patches is largely unknown. CCL20, a chemokine that recruits immature dendritic cells, is one of the few FAE-specific markers described so far. Lymphotoxin beta (LTalpha1beta2) expressed on the membrane of immune cells triggers CCL20 expression in enterocytes. In this study, we measured expression profiles of LTalpha1beta2-treated intestinal epithelial cells and selected CCL20 -coregulated genes to identify new FAE markers. METHODS: Genomic profiles of T84 and Caco-2 cell lines treated with either LTalpha1beta2, flagellin, or tumor necrosis factor alpha were measured using the Affymetrix GeneChip U133A. Clustering analysis was used to select CCL20 -coregulated genes, and laser dissection microscopy and real-time polymerase chain reaction on human biopsy specimens was used to assess the expression of the selected markers. RESULTS: Applying a 2-way analysis of variance, we identified regulated genes upon the different treatments. A subset of genes involved in inflammation and related to the nuclear factor kappaB pathway was coregulated with CCL20 . Among these genes, the antiapoptotic factor TNFAIP3 was highly expressed in the FAE. CCL23 , which was not coregulated in vitro with CCL20 , was also specifically expressed in the FAE. CONCLUSIONS: We have identified 2 novel human FAE specifically expressed genes. Most of the CCL20 -coregulated genes did not show FAE-specific expression, suggesting that other signaling pathways are critical to modulate FAE-specific gene expression.
Biological Markers, Caco-2 Cells, Chemokines, CC, Flagellin, Genomics, Humans, Intestinal Mucosa, Intracellular Signaling Peptides and Proteins, Lasers, Lymphotoxin-alpha, Lymphotoxin-beta, Membrane Proteins, Microdissection, Multigene Family, Nuclear Proteins, Phenotype, Polymerase Chain Reaction, Proteins, Tumor Necrosis Factor-alpha
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