Integrin alpha1beta1 mediates collagen induction of MMP-13 expression in MC615 chondrocytes.

Détails

ID Serval
serval:BIB_56F195FE5D3A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Integrin alpha1beta1 mediates collagen induction of MMP-13 expression in MC615 chondrocytes.
Périodique
Biochimica et Biophysica Acta
Auteur(s)
Ronzière M.C., Aubert-Foucher E., Gouttenoire J., Bernaud J., Herbage D., Mallein-Gerin F.
ISSN
0006-3002 (Print)
ISSN-L
0006-3002
Statut éditorial
Publié
Date de publication
2005
Volume
1746
Numéro
1
Pages
55-64
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
During endochondral ossification, type I collagen is synthesized by osteoblasts together with some hypertrophic chondrocytes. Type I collagen has also been reported to be progressively synthesized in degenerative joints. Because Matrix Metalloproteinase-13 (MMP-13) plays an active role in remodeling cartilage in fetal development and osteoarthritic cartilage, we investigated whether type I collagen could activate MMP-13 expression in chondrocytes. We used a well-established chondrocytic cell line (MC615) and we found that MMP-13 expression was induced in MC615 cells cultured in type I collagen gel. We also found that alpha1beta1 integrin, a major collagen receptor, was expressed by MC615 cells and we further assessed the role of alpha1beta1 integrin in conducting MMP-13 expression. Induction of MMP-13 expression by collagen was potently and synergistically inhibited by blocking antibodies against alpha1 and beta1 integrin subunits, indicating that alpha1beta1 integrin mediates the MMP-13-inducing cellular signal generated by three-dimensional type I collagen. We also determined that activities of tyrosine kinase and ERK and JNK MAP kinases were required for this collagen-induced MMP-13 expression. Interestingly, bone morphogenetic protein (BMP)-2 opposed this induction, an effect that may be related to a role of BMP-2 in the maintenance of cartilage matrix.
Mots-clé
Animals, Bone Morphogenetic Protein 2, Bone Morphogenetic Proteins/metabolism, Bone Morphogenetic Proteins/pharmacology, Cell Line, Chondrocytes/drug effects, Chondrocytes/metabolism, Collagen Type I/metabolism, Collagenases/genetics, Collagenases/metabolism, Extracellular Signal-Regulated MAP Kinases/metabolism, Gene Expression Regulation, Integrin alpha1beta1/genetics, Integrin alpha1beta1/metabolism, JNK Mitogen-Activated Protein Kinases/metabolism, Matrix Metalloproteinase 13, Mice, Phosphotyrosine/metabolism, Protein-Tyrosine Kinases/metabolism, Time Factors, Transforming Growth Factor beta/metabolism, Transforming Growth Factor beta/pharmacology
Pubmed
Open Access
Oui
Création de la notice
01/10/2015 16:28
Dernière modification de la notice
20/08/2019 15:11
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