Mitotic functions for SNAP45, a subunit of the small nuclear RNA-activating protein complex SNAPc.

Détails

Ressource 1Télécharger: 14845.pdf (916.78 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_56E189826993
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Mitotic functions for SNAP45, a subunit of the small nuclear RNA-activating protein complex SNAPc.
Périodique
Journal of Biological Chemistry
Auteur(s)
Shanmugam M., Hernandez N.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
283
Numéro
21
Pages
14845-14846
Langue
anglais
Notes
Publication types: Journal Article
Résumé
The small nuclear RNA-activating protein complex SNAP(c) is required for transcription of small nuclear RNA genes and binds to a proximal sequence element in their promoters. SNAP(c) contains five types of subunits stably associated with each other. Here we show that one of these polypeptides, SNAP45, also known as PTF delta, localizes to centrosomes during parts of mitosis, as well as to the spindle midzone during anaphase and the mid-body during telophase. Consistent with localization to these mitotic structures, both down- and up-regulation of SNAP45 lead to a G(2)/M arrest with cells displaying abnormal mitotic structures. In contrast, down-regulation of SNAP190, another SNAP(c) subunit, leads to an accumulation of cells with a G(0)/G(1) DNA content. These results are consistent with the proposal that SNAP45 plays two roles in the cell, one as a subunit of the transcription factor SNAP(c) and another as a factor required for proper mitotic progression.
Mots-clé
Antibodies/immunology, CDC2 Protein Kinase/metabolism, Centrosome/metabolism, Cyclin B/metabolism, Down-Regulation, Hela Cells, Humans, Mitosis, Protein Binding, Transcription Factors/genetics, Transcription Factors/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/06/2009 14:16
Dernière modification de la notice
20/08/2019 15:11
Données d'usage