Lipid anchoring of Arabidopsis phototropin 1 to assess the functional significance of receptor internalization: should I stay or should I go?

Détails

Ressource 1Télécharger: BIB_56A270ADA2A2.P001.pdf (25497.49 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_56A270ADA2A2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Lipid anchoring of Arabidopsis phototropin 1 to assess the functional significance of receptor internalization: should I stay or should I go?
Périodique
New Phytologist
Auteur(s)
Preuten T., Blackwood L., Christie J.M., Fankhauser C.
ISSN
1469-8137 (Electronic)
ISSN-L
0028-646X
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
206
Numéro
3
Pages
1038-1050
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The phototropin 1 (phot1) blue light receptor mediates a number of adaptive responses, including phototropism, that generally serve to optimize photosynthetic capacity. Phot1 is a plasma membrane-associated protein, but upon irradiation, a fraction is internalized into the cytoplasm. Although this phenomenon has been reported for more than a decade, its biological significance remains elusive. Here, we use a genetic approach to revisit the prevalent hypotheses regarding the functional importance of receptor internalization. Transgenic plants expressing lipidated versions of phot1 that are permanently anchored to the plasma membrane were used to analyse the effect of internalization on receptor turnover, phototropism and other phot1-mediated responses. Myristoylation and farnesylation effectively prevented phot1 internalization. Both modified photoreceptors were found to be fully functional in Arabidopsis, rescuing phototropism and all other phot1-mediated responses tested. Light-mediated phot1 turnover occurred as in the native receptor. Furthermore, our work does not provide any evidence of a role of phot1 internalization in the attenuation of receptor signalling during phototropism. Our results demonstrate that phot1 signalling is initiated at the plasma membrane. They furthermore indicate that release of phot1 into the cytosol is not linked to receptor turnover or desensitization.
Mots-clé
Arabidopsis/chemistry, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Cell Membrane/metabolism, Lipid-Linked Proteins/physiology, Phosphoproteins/genetics, Phosphoproteins/metabolism, Phototropins/genetics, Phototropins/metabolism, Phototropism/genetics, Plants, Genetically Modified/metabolism, Plants, Genetically Modified/radiation effects, Signal Transduction
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/05/2015 10:00
Dernière modification de la notice
20/08/2019 15:10
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