Article: article from journal or magazin.
Characterization of a ribosomal RNA gene cluster from Clostridium tyrobutyricum: phylogenetic positioning based on the 16S and 23S nucleotide sequences.
Systematic and Applied Microbiology
A ribosomal operon of Clostridium tyrobutyricum NIZO strain 51 was cloned into plasmid vectors and characterized by DNA sequencing of the 16S rRNA, 23S rRNA and part of the 5S rRNA. The organization of this ribosomal operon of C. tyrobutyricum is similar to that of C. perfringens and Bacillus subtilis. A proposal was made for the processing stems and the sites of maturation of 16S and 23S rRNA. The phylogenetic position of C. tyrobutyricum was reconstructed by comparative 16S rRNA and 23S rRNA sequence analyses. Both analysis provided a similar position for C. tyrobutyricum. The 16S rRNA comparisons showed that C. kluyveri and C. ljungdahlii are the closest relatives of C. tyrobutyricum.
Clostridium tyrobutyricum, 16S rRNA, 23S rRNA, Phylogeny
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