Comparison of PCR-based DNA fingerprinting techniques for the identification of Listeria species and their use for atypical Listeria isolates

Details

Serval ID
serval:BIB_55F90449EF3F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparison of PCR-based DNA fingerprinting techniques for the identification of Listeria species and their use for atypical Listeria isolates
Journal
International Journal of Systematic Bacteriology
Author(s)
Vaneechoutte  M., Boerlin  P., Tichy  H. V., Bannerman  E., Jager  B., Bille  J.
ISSN
0020-7713
Publication state
Published
Issued date
01/1998
Peer-reviewed
Oui
Volume
48 Pt 1
Pages
127-39
Notes
Comparative Study
Journal Article --- Old month value: Jan
Abstract
Four PCR-based DNA fingerprinting techniques were compared for their ability to identify at the species level a heterogeneous collection of isolates belonging to the six valid Listeria species. 16S rDNA-RFLP analysis identified all species and 16S rDNA-SSCP analysis identified almost all species. Also, isolates with unusual biochemical characteristics and/or unusual antigenic composition could be identified correctly. rRNA-intracistronic length polymorphism analysis suffered from high intraspecific variability, a limited number of fragments per profile, and small length differences between the spacers of different species. tRNA-intergenic length polymorphism analysis resulted in identification of all isolates but one, when fluorescent DNA capillary electrophoresis was used such that fragment length differences of 1 bp could be resolved. The four techniques yielded comparable results relevant to the taxonomy of Listeria. They all indicate a high degree of genetic relatedness between L. innocua and L. welshimeri, homogeneity of L. grayi, distinct but clear relatedness of L. grayi to the other Listeria species, a clear distinction between the two subspecies of L. ivanovii, and a clear distinction between Listeria isolates and isolates from closely related taxa or from species which are phenotypically difficult to distinguish from Listeria. New sequence determination of the 16S rRNA gene was necessary to obtain sequences in accordance with the findings of 16S rDNA-RFLP analysis.
Keywords
DNA Fingerprinting/*methods DNA, Bacterial/analysis Genes, Bacterial/genetics Listeria monocytogenes/*classification/*genetics/isolation & purification Molecular Sequence Data Phylogeny Polymerase Chain Reaction/methods Polymorphism, Restriction Fragment Length Polymorphism, Single-Stranded Conformational RNA, Bacterial/analysis RNA, Ribosomal, 16S/analysis RNA, Transfer/analysis
Pubmed
Web of science
Open Access
Yes
Create date
11/02/2008 12:39
Last modification date
20/08/2019 14:10
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