Atrophins are corepressors for NR2E3

Details

Serval ID
serval:BIB_558F89CF88AA
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Poster: Summary – with images – on one page of the results of a researche project. The summaries of the poster must be entered in "Abstract" and not "Poster".
Collection
Publications
Institution
Title
Atrophins are corepressors for NR2E3
Title of the conference
Atrophins are corepressors for NR2E3
Author(s)
Escher P., Roduit R., Favez T., Voirol N., Chen S., Tsai C.C., Schorderet D.F.
Publisher
Investigate ophtalmology and visual science IOVS
Organization
ARVO Annual Meeting 2007
Address
Fort Lauderdale, Florida, USA
Publication state
Published
Issued date
05/2007
Peer-reviewed
Oui
Volume
48
Number
48
Language
english
Abstract
Purpose:: Photoreceptor cell fate is determined by a complex transcriptional regulatory system. Whereas many transcription factors are involved in retinal progenitor cell proliferation, the cone-rod homeobox protein CRX appears to be a master regulator of the commitment of retinal progenitors towards the photoreceptor lineage. A current model proposes that in rods CRX interacts with NRL (neural retina-specific leucine zipper protein) to specify a rod-specific gene expression. The photoreceptor-selective nuclear hormone receptor NR2E3 is hypothesized to repress the CRX/NRL-mediated transcription of cone-specific genes by interacting with corepressors. Importance of this repression is exemplified by the association of mutations in nr2e3 with enhanced S-cone syndrome (ESCS). We aim at identifying candidate corepressors for NR2E3-mediated transrepression.
Methods:: By cell culture-based transactivation assays in 293T cells, we test the corepressor activity of atrophin-1, atrophin-2 and NCoR on rhodopsin and M-opsin promoters, cotransfecting CRX and NRL, together with NR2E3 wild-type and mutant proteins. Molecular interactions are further tested by co-immunoprecipitation, chromatin-immunoprecipitation, gel-retardation assays, BRET and in situ hybridization.
Results:: Both atrophin-1 and atrophin-2 proteins mediate repression of the M-opsin promoter in the presence of NR2E3. Transactivation mediated by CRX alone is also repressed by atrophins, whereas that of NRL is not. Interestingly, NR2E3R311Q, the most common mutation found in ESCS patients, shows impaired repression when coexpressed with atrophins in our transactivation assays.
Conclusions:: In addition to the reported interaction of atrophins with NR2E1, we show that this class of corepressors is also interacting with NR2E3. The differential repression mediated by atrophins with wild-type and mutant NR2E3 strongly suggests a physiological relevance of NR2E3/atrophin interactions in photoreceptors in vivo.

Keywords
Purpose:: Photoreceptor cell fate is determined by a complex transcriptional regulatory system. Whereas many transcription factors are involved in retinal progenitor cell proliferation, the cone-rod homeobox protein CRX appears to be a master regulator of the commitment of retinal progenitors towards the photoreceptor lineage. A current model proposes that in rods CRX interacts with NRL (neural retina-specific leucine zipper protein) to specify a rod-specific gene expression. The photoreceptor-selective nuclear hormone receptor NR2E3 is hypothesized to repress the CRX/NRL-mediated transcription of cone-specific genes by interacting with corepressors. Importance of this repression is exemplified by the association of mutations in nr2e3 with enhanced S-cone syndrome (ESCS). We aim at identifying candidate corepressors for NR2E3-mediated transrepression.
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19/09/2017 12:25
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20/08/2019 14:10
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