Human-induced pluripotent stem cell-derived cardiomyocytes from cardiac progenitor cells: effects of selective ion channel blockade.

Détails

ID Serval
serval:BIB_5561847D27BB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human-induced pluripotent stem cell-derived cardiomyocytes from cardiac progenitor cells: effects of selective ion channel blockade.
Périodique
Europace
Auteur(s)
Altomare C., Pianezzi E., Cervio E., Bolis S., Biemmi V., Benzoni P., Camici G.G., Moccetti T., Barile L., Vassalli G.
ISSN
1532-2092 (Electronic)
ISSN-L
1099-5129
Statut éditorial
Publié
Date de publication
12/2016
Peer-reviewed
Oui
Volume
18
Numéro
suppl 4
Pages
iv67-iv76
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes are likely to revolutionize electrophysiological approaches to arrhythmias. Recent evidence suggests the somatic cell origin of hiPSCs may influence their differentiation potential. Owing to their cardiomyogenic potential, cardiac-stromal progenitor cells (CPCs) are an interesting cellular source for generation of hiPSC-derived cardiomyocytes. The effect of ionic current blockade in hiPSC-derived cardiomyocytes generated from CPCs has not been characterized yet.
Human-induced pluripotent stem cell-derived cardiomyocytes were generated from adult CPCs and skin fibroblasts from the same individuals. The effect of selective ionic current blockade on spontaneously beating hiPSC-derived cardiomyocytes was assessed using multi-electrode arrays. Cardiac-stromal progenitor cells could be reprogrammed into hiPSCs, then differentiated into hiPSC-derived cardiomyocytes. Human-induced pluripotent stem cell-derived cardiomyocytes of cardiac origin showed higher upregulation of cardiac-specific genes compared with those of fibroblastic origin. Human-induced pluripotent stem cell-derived cardiomyocytes of both somatic cell origins exhibited sensitivity to tetrodotoxin, a blocker of Na(+ )current (INa), nifedipine, a blocker of L-type Ca(2+ )current (ICaL), and E4031, a blocker of the rapid component of delayed rectifier K(+ )current (IKr). Human-induced pluripotent stem cell-derived cardiomyocytes of cardiac origin exhibited sensitivity to JNJ303, a blocker of the slow component of delayed rectifier K(+ )current (IKs).
In hiPSC-derived cardiomyocytes of cardiac origin, INa, ICaL, IKr, and IKs were present as tetrodotoxin-, nifedipine-, E4031-, and JNJ303-sensitive currents, respectively. Although cardiac differentiation efficiency was improved in hiPSCs of cardiac vs. non-cardiac origin, no major functional differences were observed between hiPSC-derived cardiomyocytes of different somatic cell origins. Further studies are warranted to characterize electrophysiological properties of hiPSC-derived cardiomyocytes generated from CPCs.

Mots-clé
Calcium Channel Blockers/pharmacology, Calcium Channels, L-Type/drug effects, Calcium Channels, L-Type/metabolism, Cell Differentiation, Cell Lineage, Cells, Cultured, Cellular Reprogramming, Delayed Rectifier Potassium Channels/antagonists & inhibitors, Delayed Rectifier Potassium Channels/metabolism, Fibroblasts/drug effects, Fibroblasts/metabolism, Humans, Induced Pluripotent Stem Cells/drug effects, Induced Pluripotent Stem Cells/metabolism, Membrane Potentials, Membrane Transport Modulators/pharmacology, Myocytes, Cardiac/drug effects, Myocytes, Cardiac/metabolism, Phenotype, Potassium Channel Blockers/pharmacology, Sodium Channel Blockers/pharmacology, Sodium Channels/drug effects, Sodium Channels/metabolism, Arrhythmia, Cardiac progenitor cell, Cardiomyocyte, Induced pluripotent stem cell, Ion current
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/01/2017 20:24
Dernière modification de la notice
20/08/2019 15:10
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