Robust synchronization of coupled circadian and cell cycle oscillators in single mammalian cells.

Détails

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Version: de l'auteur
ID Serval
serval:BIB_54F704C349C4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Robust synchronization of coupled circadian and cell cycle oscillators in single mammalian cells.
Périodique
Molecular Systems Biology
Auteur(s)
Bieler J., Cannavo R., Gustafson K., Gobet C., Gatfield D., Naef F.
ISSN
1744-4292 (Electronic)
ISSN-L
1744-4292
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
10
Numéro
7
Pages
739
Langue
anglais
Résumé
Circadian cycles and cell cycles are two fundamental periodic processes with a period in the range of 1 day. Consequently, coupling between such cycles can lead to synchronization. Here, we estimated the mutual interactions between the two oscillators by time-lapse imaging of single mammalian NIH3T3 fibroblasts during several days. The analysis of thousands of circadian cycles in dividing cells clearly indicated that both oscillators tick in a 1:1 mode-locked state, with cell divisions occurring tightly 5 h before the peak in circadian Rev-Erbα-YFP reporter expression. In principle, such synchrony may be caused by either unidirectional or bidirectional coupling. While gating of cell division by the circadian cycle has been most studied, our data combined with stochastic modeling unambiguously show that the reverse coupling is predominant in NIH3T3 cells. Moreover, temperature, genetic, and pharmacological perturbations showed that the two interacting cellular oscillators adopt a synchronized state that is highly robust over a wide range of parameters. These findings have implications for circadian function in proliferative tissues, including epidermis, immune cells, and cancer.
Mots-clé
cell cycle, circadian cycle, single cells, synchronization, time-lapse imaging
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/07/2014 11:21
Dernière modification de la notice
08/05/2019 18:46
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