Quantification of stored red blood cell fluctuations by time-lapse holographic cell imaging.

Détails

ID Serval
serval:BIB_54F502EC93B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Quantification of stored red blood cell fluctuations by time-lapse holographic cell imaging.
Périodique
Biomedical optics express
Auteur(s)
Jaferzadeh K., Moon I., Bardyn M., Prudent M., Tissot J.D., Rappaz B., Javidi B., Turcatti G., Marquet P.
ISSN
2156-7085 (Print)
ISSN-L
2156-7085
Statut éditorial
Publié
Date de publication
01/10/2018
Peer-reviewed
Oui
Volume
9
Numéro
10
Pages
4714-4729
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
We propose methods to quantitatively calculate the fluctuation rate of red blood cells with nanometric axial and millisecond temporal sensitivity at the single-cell level by using time-lapse holographic cell imaging. For this quantitative analysis, cell membrane fluctuations (CMFs) were measured for RBCs stored at different storage times. Measurements were taken over the whole membrane for both the ring and dimple sections separately. The measurements show that healthy RBCs that maintain their discocyte shape become stiffer with storage time. The correlation analysis demonstrates a significant negative correlation between CMFs and the sphericity coefficient, which characterizes the morphological type of erythrocyte. In addition, we show the correlation results between CMFs and other morphological properties such as projected surface area, surface area, mean corpuscular volume, and mean corpuscular hemoglobin.
Mots-clé
Biotechnology, Atomic and Molecular Physics, and Optics
Pubmed
Web of science
Création de la notice
04/10/2018 13:08
Dernière modification de la notice
20/08/2019 15:09
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