Drosophila anterior-posterior polarity requires actin-dependent PAR-1 recruitment to the oocyte posterior.

Détails

ID Serval
serval:BIB_54E29DB366DA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Drosophila anterior-posterior polarity requires actin-dependent PAR-1 recruitment to the oocyte posterior.
Périodique
Current Biology
Auteur(s)
Doerflinger H., Benton R., Torres I.L., Zwart M.F., St Johnston D.
ISSN
0960-9822[print], 0960-9822[linking]
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
16
Numéro
11
Pages
1090-1095
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The Drosophila anterior-posterior axis is established at stage 7 of oogenesis when the posterior follicle cells signal to polarize the oocyte microtubule cytoskeleton. This requires the conserved PAR-1 kinase, which can be detected at the posterior of the oocyte in immunostainings from stage 9. However, this localization depends on Oskar localization, which requires the earlier PAR-1-dependent microtubule reorganization, indicating that Oskar-associated PAR-1 cannot establish oocyte polarity. Here we analyze the function of the different PAR-1 isoforms and find that only PAR-1 N1 isoforms can completely rescue the oocyte polarity phenotype. Furthermore, PAR-1 N1 is recruited to the posterior cortex of the oocyte at stage 7 in response to the polarizing follicle cell signal, and this requires actin, but not microtubules. This suggests that posterior PAR-1 N1 polarizes the microtubule cytoskeleton. PAR-1 N1 localization is mediated by a cortical targeting domain and a conserved anterior-lateral exclusion signal in its C-terminal linker domain. PAR-1 is also required for the polarization of the C. elegans zygote and is recruited to the posterior cortex in an actin-dependent manner. Our results therefore identify a molecular parallel between axis formation in Drosophila and C. elegans and make Drosophila PAR-1 N1 the earliest known marker for the polarization of the oocyte.
Mots-clé
Actins/metabolism, Actins/physiology, Amino Acid Sequence, Animals, Body Patterning/physiology, Caenorhabditis elegans/growth &amp, development, Caenorhabditis elegans/metabolism, Conserved Sequence, Drosophila/enzymology, Drosophila/genetics, Drosophila Proteins/analysis, Drosophila Proteins/chemistry, Microtubules/metabolism, Molecular Sequence Data, Oocytes/cytology, Oocytes/enzymology, Protein Isoforms/analysis, Protein Isoforms/chemistry, Protein Kinases/analysis, Protein Kinases/chemistry, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases, Recombinant Fusion Proteins/analysis
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/03/2008 10:36
Dernière modification de la notice
20/08/2019 14:09
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