Cell wall maturation of Arabidopsis trichomes is dependent on exocyst subunit EXO70H4 and involves callose deposition.

Détails

ID Serval
serval:BIB_545C533C0ABE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cell wall maturation of Arabidopsis trichomes is dependent on exocyst subunit EXO70H4 and involves callose deposition.
Périodique
Plant Physiology
Auteur(s)
Kulich I., Vojtíková Z., Glanc M., Ortmannová J., Rasmann S., Žárský V.
ISSN
1532-2548 (Electronic)
ISSN-L
0032-0889
Statut éditorial
Publié
Date de publication
2015
Volume
168
Numéro
1
Pages
120-131
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Arabidopsis (Arabidopsis thaliana) leaf trichomes are single-cell structures with a well-studied development, but little is understood about their function. Developmental studies focused mainly on the early shaping stages, and little attention has been paid to the maturation stage. We focused on the EXO70H4 exocyst subunit, one of the most up-regulated genes in the mature trichome. We uncovered EXO70H4-dependent development of the secondary cell wall layer, highly autofluorescent and callose rich, deposited only in the upper part of the trichome. The boundary is formed between the apical and the basal parts of mature trichome by a callose ring that is also deposited in an EXO70H4-dependent manner. We call this structure the Ortmannian ring (OR). Both the secondary cell wall layer and the OR are absent in the exo70H4 mutants. Ecophysiological aspects of the trichome cell wall thickening include interference with antiherbivore defense and heavy metal accumulation. Ultraviolet B light induces EXO70H4 transcription in a CONSTITUTIVE PHOTOMORPHOGENIC1-dependent way, resulting in stimulation of trichome cell wall thickening and the OR biogenesis. EXO70H4-dependent trichome cell wall hardening is a unique phenomenon, which may be conserved among a variety of the land plants. Our analyses support a concept that Arabidopsis trichome is an excellent model to study molecular mechanisms of secondary cell wall deposition.
Mots-clé
Arabidopsis/metabolism, Arabidopsis/radiation effects, Arabidopsis Proteins/metabolism, Cell Wall/metabolism, Cell Wall/ultrastructure, Copper/metabolism, Cytokinesis/radiation effects, Fluorescence, Glucans/metabolism, Mutation/genetics, Protein Subunits/metabolism, Trichomes/metabolism, Trichomes/radiation effects, Ultraviolet Rays, Vesicular Transport Proteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/07/2015 8:10
Dernière modification de la notice
20/08/2019 15:09
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