Exon junction complex subunits are required to splice Drosophila MAP kinase, a large heterochromatic gene.

Détails

ID Serval
serval:BIB_54231003ECAE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Exon junction complex subunits are required to splice Drosophila MAP kinase, a large heterochromatic gene.
Périodique
Cell
Auteur(s)
Roignant J.Y., Treisman J.E.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Statut éditorial
Publié
Date de publication
15/10/2010
Peer-reviewed
Oui
Volume
143
Numéro
2
Pages
238-250
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural
Publication Status: ppublish
Résumé
The exon junction complex (EJC) is assembled on spliced mRNAs upstream of exon-exon junctions and can regulate their subsequent translation, localization, or degradation. We isolated mutations in Drosophila mago nashi (mago), which encodes a core EJC subunit, based on their unexpectedly specific effects on photoreceptor differentiation. Loss of Mago prevents epidermal growth factor receptor signaling, due to a large reduction in MAPK mRNA levels. MAPK expression also requires the EJC subunits Y14 and eIF4AIII and EJC-associated splicing factors. Mago depletion does not affect the transcription or stability of MAPK mRNA but alters its splicing pattern. MAPK expression from an exogenous promoter requires Mago only when the template includes introns. MAPK is the primary functional target of mago in eye development; in cultured cells, Mago knockdown disproportionately affects other large genes located in heterochromatin. These data support a nuclear role for EJC components in splicing a specific subset of introns.
Mots-clé
Animals, Cell Differentiation, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Drosophila melanogaster/embryology, Drosophila melanogaster/genetics, Drosophila melanogaster/metabolism, ErbB Receptors/genetics, Exons, Eye/embryology, Gene Knockdown Techniques, Heterochromatin/metabolism, Humans, Mitogen-Activated Protein Kinases/genetics, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Photoreceptor Cells, Invertebrate/cytology, RNA Splicing, Signal Transduction, Wings, Animal/embryology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/10/2019 12:58
Dernière modification de la notice
29/10/2019 6:26
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