Successful DNA typing of ultrafiltered urines used to detect EPO doping.
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serval:BIB_540222566C48
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Successful DNA typing of ultrafiltered urines used to detect EPO doping.
Journal
Forensic Science International. Genetics
ISSN
1878-0326
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
1
Number
3-4
Pages
281-282
Language
english
Abstract
Endogenous and exogenous erythropoietin (EPO) present in urine can be distinguished according to their isoelectric profiles. This methodology requires urine samples to be concentrated about 200 to 1000 times with manipulations that should remove most of the cells occurring in the original sample. In this study, we tried to obtain DNA profiles from 10 ultrafiltered urines (retentates) in order to evaluate whether a formal genetic identification was technically feasible. No nuclear DNA profiles could be established from retentates, despite 34 PCR-cycles amplifications. Contrastingly, mitochondrial DNA (mtDNA) profiles were obtained for 9 out of the 10 retentates. Apart from some particularities, retentate mtDNA profiles were all distinct and matched mtDNA profiles of corresponding reference samples.
Keywords
DNA, DNA Fingerprinting, DNA, Mitochondrial, Doping in Sports, Erythropoietin, Erythropoietin, Recombinant, Female, Forensic Genetics, Humans, Male, Ultrafiltration
Pubmed
Web of science
Create date
22/02/2008 15:54
Last modification date
20/08/2019 14:09
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