The general transcription factors IIA, IIB, IIF, and IIE are required for RNA polymerase II transcription from the human U1 small nuclear RNA promoter.

Détails

ID Serval
serval:BIB_537A0734F4F2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The general transcription factors IIA, IIB, IIF, and IIE are required for RNA polymerase II transcription from the human U1 small nuclear RNA promoter.
Périodique
Molecular and Cellular Biology
Auteur(s)
Kuhlman T.C., Cho H., Reinberg D., Hernandez N.
ISSN
0270-7306[print], 0270-7306[linking]
Statut éditorial
Publié
Date de publication
03/1999
Volume
19
Numéro
3
Pages
2130-2141
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
RNA polymerase II transcribes the mRNA-encoding genes and the majority of the small nuclear RNA (snRNA) genes. The formation of a minimal functional transcription initiation complex on a TATA-box-containing mRNA promoter has been well characterized and involves the ordered assembly of a number of general transcription factors (GTFs), all of which have been either cloned or purified to near homogeneity. In the human RNA polymerase II snRNA promoters, a single element, the proximal sequence element (PSE), is sufficient to direct basal levels of transcription in vitro. The PSE is recognized by the basal transcription complex SNAPc. SNAPc, which is not required for transcription from mRNA-type RNA polymerase II promoters such as the adenovirus type 2 major late (Ad2ML) promoter, is thought to recruit TATA binding protein (TBP) and nucleate the assembly of the snRNA transcription initiation complex, but little is known about which GTFs other than TBP are required. Here we show that the GTFs IIA, IIB, IIF, and IIE are required for efficient RNA polymerase II transcription from snRNA promoters. Thus, although the factors that recognize the core elements of RNA polymerase II mRNA and snRNA-type promoters differ, they mediate the recruitment of many common GTFs.
Mots-clé
Animals, Hela Cells, Humans, Promoter Regions, Genetic, RNA Polymerase II/genetics, RNA Polymerase III/genetics, RNA, Small Nuclear, Rabbits, Ribonucleoprotein, U1 Small Nuclear/genetics, Ribonucleoprotein, U4-U6 Small Nuclear/genetics, Transcription Factor TFIIA, Transcription Factor TFIIB, Transcription Factors/genetics, Transcription Factors/metabolism, Transcription Factors, TFII, Transcription, Genetic
Pubmed
Web of science
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
20/08/2019 15:08
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