In vivo measurements of change in tissue oxygen level during irradiation reveal novel dose rate dependence.
Details
Serval ID
serval:BIB_533C949EDC61
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vivo measurements of change in tissue oxygen level during irradiation reveal novel dose rate dependence.
Journal
Radiotherapy and oncology
ISSN
1879-0887 (Electronic)
ISSN-L
0167-8140
Publication state
In Press
Peer-reviewed
Oui
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Publication Status: aheadofprint
Abstract
This study aimed to investigate the radiochemical oxygen depletion (ROD) in vivo by directly measuring oxygen levels in various mouse tissues during ultra-high dose rate (UHDR) irradiation at clinically relevant doses and dose rates.
Mice bearing subcutaneous human glioblastoma (U-87 MG) tumors were used for tumor and normal tissue (skin, muscle, brain) measurements. An oxygen-sensitive phosphorescent probe (Oxyphor PtG4) was injected into the tissues, and oxygen levels were monitored using a fiberoptic phosphorometer during UHDR irradiation with a 6 MeV electron linear accelerator (LINAC). Dose escalation experiments (10-40 Gy) were performed at a dose rate of 1300 Gy/s, and dose rate escalation experiments were conducted at a fixed dose of 40 Gy with dose rates ranging from 2 to 101 Gy/s.
Radiation-induced change in tissue oxygenation (ΔpO <sub>2</sub> ) increased linearly with dose and correlated with baseline tissue oxygenation levels in the range of 0 - 30 mmHg. At higher baseline tissue oxygenation levels, such as those observed in muscle and brain, there was no corresponding increase in ΔpO <sub>2</sub> . When we modulated dose rate, ΔpO <sub>2</sub> increased steeply up to ∼ 20 Gy/s and plateaued thereafter. The relationship between ΔpO <sub>2</sub> and dose rate showcases the interplay between ROD and reoxygenation.
While UHDR irradiation induces measurable oxygen depletion in tissues, the observed changes in oxygenation levels do not support the hypothesis that ROD-induced radioresistance is responsible for the FLASH tissue-sparing effect at clinically relevant doses and dose rates. These findings highlight the need for further investigation into alternative mechanisms underlying the FLASH effect.
Mice bearing subcutaneous human glioblastoma (U-87 MG) tumors were used for tumor and normal tissue (skin, muscle, brain) measurements. An oxygen-sensitive phosphorescent probe (Oxyphor PtG4) was injected into the tissues, and oxygen levels were monitored using a fiberoptic phosphorometer during UHDR irradiation with a 6 MeV electron linear accelerator (LINAC). Dose escalation experiments (10-40 Gy) were performed at a dose rate of 1300 Gy/s, and dose rate escalation experiments were conducted at a fixed dose of 40 Gy with dose rates ranging from 2 to 101 Gy/s.
Radiation-induced change in tissue oxygenation (ΔpO <sub>2</sub> ) increased linearly with dose and correlated with baseline tissue oxygenation levels in the range of 0 - 30 mmHg. At higher baseline tissue oxygenation levels, such as those observed in muscle and brain, there was no corresponding increase in ΔpO <sub>2</sub> . When we modulated dose rate, ΔpO <sub>2</sub> increased steeply up to ∼ 20 Gy/s and plateaued thereafter. The relationship between ΔpO <sub>2</sub> and dose rate showcases the interplay between ROD and reoxygenation.
While UHDR irradiation induces measurable oxygen depletion in tissues, the observed changes in oxygenation levels do not support the hypothesis that ROD-induced radioresistance is responsible for the FLASH tissue-sparing effect at clinically relevant doses and dose rates. These findings highlight the need for further investigation into alternative mechanisms underlying the FLASH effect.
Pubmed
Web of science
Open Access
Yes
Create date
25/09/2024 10:16
Last modification date
31/10/2024 7:13