Effects of alpha-phosphoglucomutase deficiency on cell wall properties and fitness in Streptococcus gordonii.

Details

Serval ID
serval:BIB_530C3E6CA1C0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of alpha-phosphoglucomutase deficiency on cell wall properties and fitness in Streptococcus gordonii.
Journal
Microbiology
Author(s)
Bizzini A., Majcherczyk P., Beggah-Möller S., Soldo B., Entenza J.M., Gaillard M., Moreillon P., Lazarevic V.
ISSN
1350-0872[print], 1350-0872[linking]
Publication state
Published
Issued date
2007
Volume
153
Number
Pt 2
Pages
490-498
Language
english
Abstract
Streptococcus gordonii alpha-phosphoglucomutase, which converts glucose 6-phosphate to glucose 1-phosphate, is encoded by pgm. The pgm transcript is monocistronic and is initiated from a sigma(A)-like promoter. Mutants with a gene disruption in pgm exhibited an altered cell wall muropeptide pattern and a lower teichoic acid content, and had reduced fitness both in vitro and in vivo. In vitro, the reduced fitness included reduced growth, reduced viability in the stationary phase and increased autolytic activity. In vivo, the pgm-deficient strain had a lower virulence in a rat model of experimental endocarditis.
Keywords
Base Sequence, Cell Wall/chemistry, Cell Wall/metabolism, Endocarditis, Bacterial/microbiology, Endocarditis, Bacterial/physiopathology, Gene Deletion, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Phosphoglucomutase/chemistry, Phosphoglucomutase/genetics, Sequence Analysis, DNA, Streptococcal Infections/microbiology, Streptococcal Infections/physiopathology, Streptococcus/enzymology, Streptococcus/genetics, Virulence
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:45
Last modification date
20/08/2019 14:08
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