The repressor element silencing transcription factor (REST)-mediated transcriptional repression requires the inhibition of Sp1.

Détails

ID Serval
serval:BIB_5233C37B0D56
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The repressor element silencing transcription factor (REST)-mediated transcriptional repression requires the inhibition of Sp1.
Périodique
Journal of Biological Chemistry
Auteur(s)
Plaisance V., Niederhauser G., Azzouz F., Lenain V., Haefliger J.A., Waeber G., Abderrahmani A.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
280
Numéro
1
Pages
401-407
Langue
anglais
Notes
Publication types: Journal Article
Résumé
The terminal differentiation of neuronal and pancreatic beta-cells requires the specific expression of genes that are targets of an important transcriptional repressor named RE-1 silencing transcription factor (REST). The molecular mechanism by which these REST target genes are expressed only in neuronal and beta-cells and are repressed by REST in other tissues is a central issue in differentiation program of neuronal and beta-cells. Herein, we showed that the transcriptional factor Sp1 was required for expression of most REST target genes both in insulin-secreting cells and neuronal-like cells where REST is absent. Inhibition of REST in a non-beta and a non-neuronal cell model restored the transcriptional activity of Sp1. This activity was also restored by trichostatin A indicating the requirement of histone deacetylases for the REST-mediated silencing of Sp1. Conversely, exogenous introduction of REST blocked Sp1-mediated transcriptional activity. The REST inhibitory effect was mediated through its C-terminal repressor domain, which could interact with Sp1. Taken together, these data show that the inhibition of Sp1 by REST is required for the silencing of its target genes expression in non-neuronal and in non-beta-cells. We conclude that the interplay between REST and Sp1 determines the cell-specific expression of REST target genes.
Mots-clé
Animals, Cell Differentiation/genetics, Gene Expression Regulation, Gene Silencing, Hela Cells, Humans, Islets of Langerhans/cytology, Islets of Langerhans/physiology, Mice, Neurons/cytology, Neurons/physiology, PC12 Cells, Rats, Repressor Proteins/genetics, Sp1 Transcription Factor/antagonists & inhibitors, Sp1 Transcription Factor/genetics, Transcription Factors/genetics, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:16
Dernière modification de la notice
20/08/2019 15:07
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