The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation

Détails

ID Serval
serval:BIB_5232DDE07419
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation
Périodique
European Journal of Biochemistry
Auteur(s)
Clement  S., Tasinato  A., Boscoboinik  D., Azzi  A.
ISSN
0014-2956 (Print)
Statut éditorial
Publié
Date de publication
06/1997
Volume
246
Numéro
3
Pages
745-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 15
Résumé
Previous work had established that, in smooth muscle cells, alpha-tocopherol negatively regulates protein kinase C by preventing its activation [Tasinato, A., Boscoboinik, D., Bartoli, G. M., Maroni, P. & Azzi, A. (1995) Proc. Natl Acad. Sci. USA 92, 12190-12194]. In this study, the mechanism by which this event takes place has been analyzed. The regulation by alpha-tocopherol of protein kinase C expression, activity and phosphorylation has been followed during the synthesis of protein kinase C after its down-regulation by phorbol 12-myristate 13-acetate. The data show that protein kinase C isoenzyme alpha is synthesised significantly more (30% 72 h after down-regulation) in the presence of alpha-tocopherol. However, its activity is significantly less (45% diminution) and its phosphorylation state is also decreased (60% diminution). The effect of alpha-tocopherol appears not to be shared by the analogue beta-tocopherol, provided with similar radical-scavenging properties. The data are interpreted in terms of a diminution of protein kinase C phosphorylation, specifically caused by alpha-tocopherol, resulting in a decreased enzyme specific activity.
Mots-clé
Animals Down-Regulation/*drug effects Isoenzymes/*biosynthesis/metabolism Muscle, Smooth/*enzymology Phosphorylation Protein Binding Protein Kinase C/*biosynthesis/metabolism Protein Kinase C-alpha Rats Tetradecanoylphorbol Acetate/*pharmacology Vitamin E/*pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2008 9:44
Dernière modification de la notice
08/05/2019 18:37
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