The Cockayne syndrome B protein, involved in transcription-coupled DNA repair, resides in an RNA polymerase II-containing complex

Détails

ID Serval
serval:BIB_5221B8EB0D97
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The Cockayne syndrome B protein, involved in transcription-coupled DNA repair, resides in an RNA polymerase II-containing complex
Périodique
EMBO Journal
Auteur(s)
van Gool  A. J., Citterio  E., Rademakers  S., van Os  R., Vermeulen  W., Constantinou  A., Egly  J. M., Bootsma  D., Hoeijmakers  J. H.
ISSN
0261-4189 (Print)
Statut éditorial
Publié
Date de publication
10/1997
Volume
16
Numéro
19
Pages
5955-65
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 1
Résumé
Transcription-coupled repair (TCR), a subpathway of nucleotide excision repair (NER) defective in Cockayne syndrome A and B (CSA and CSB), is responsible for the preferential removal of DNA lesions from the transcribed strand of active genes, permitting rapid resumption of blocked transcription. Here we demonstrate by microinjection of antibodies against CSB and CSA gene products into living primary fibroblasts, that both proteins are required for TCR and for recovery of RNA synthesis after UV damage in vivo but not for basal transcription itself. Furthermore, immunodepletion showed that CSB is not required for in vitro NER or transcription. Its central role in TCR suggests that CSB interacts with other repair and transcription proteins. Gel filtration of repair- and transcription-competent whole cell extracts provided evidence that CSB and CSA are part of large complexes of different sizes. Unexpectedly, there was no detectable association of CSB with several candidate NER and transcription proteins. However, a minor but significant portion (10-15%) of RNA polymerase II was found to be tightly associated with CSB. We conclude that within cell-free extracts, CSB is not stably associated with the majority of core NER or transcription components, but is part of a distinct complex involving RNA polymerase II. These findings suggest that CSB is implicated in, but not essential for, transcription, and support the idea that Cockayne syndrome is due to a combined repair and transcription deficiency.
Mots-clé
Cockayne Syndrome/*genetics Consensus Sequence DNA Helicases/genetics/metabolism/*physiology *DNA Repair DNA Repair Enzymes Hela Cells Humans Macromolecular Substances Proteins/metabolism RNA Polymerase II/*metabolism Transcription Factors *Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 15:50
Dernière modification de la notice
03/03/2018 17:12
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