pRB binds to and modulates the transrepressing activity of the E1A-regulated transcription factor p120E4F.

Détails

ID Serval
serval:BIB_52076D894A81
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
pRB binds to and modulates the transrepressing activity of the E1A-regulated transcription factor p120E4F.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Fajas L., Paul C., Zugasti O., Le Cam L., Polanowska J., Fabbrizio E., Medema R., Vignais M.L., Sardet C.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2000
Volume
97
Numéro
14
Pages
7738-7743
Langue
anglais
Résumé
The retinoblastoma protein pRB is involved in the transcriptional control of genes essential for cell cycle progression and differentiation. pRB interacts with different transcription factors and thereby modulates their activity by sequestration, corepression, or activation. We report that pRB, but not p107 and p130, binds to and facilitates repression by p120(E4F), a ubiquitously expressed GLI-Kruppel-related protein identified as a cellular target of E1A. The interaction involves two distinct regions of p120(E4F) and the C-terminal part of pRB. In vivo pRB-p120(E4F) complexes can only be detected in growth-arrested cells, and accordingly contain the hypophosphorylated form of pRB. Repression of an E4F-responsive promoter is strongly increased by combined expression of p120(E4F) and pRB, which correlates with pRB-dependent enhancement of p120(E4F) binding activity. Elevated levels of p120(E4F) have been shown to block growth of mouse fibroblasts in G(1). We find this requires pRB, because RB(-/-) fibroblasts are significantly less sensitive to excess p120(E4F).
Mots-clé
Adenovirus E1A Proteins/metabolism, Adenovirus E4 Proteins/genetics, Adenovirus E4 Proteins/metabolism, Animals, Binding Sites, Cell Division, Growth Inhibitors, Mice, Mutation, Protein Binding, Repressor Proteins/genetics, Repressor Proteins/metabolism, Retinoblastoma Protein/metabolism, Zinc Fingers
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/03/2013 17:08
Dernière modification de la notice
08/05/2019 18:36
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