Thyrotropin-releasing hormone-stimulated thyrotropin expression involves islet-brain-1/c-Jun N-terminal kinase interacting protein-1.

Détails

ID Serval
serval:BIB_51FE0D732A62
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Thyrotropin-releasing hormone-stimulated thyrotropin expression involves islet-brain-1/c-Jun N-terminal kinase interacting protein-1.
Périodique
Endocrinology
Auteur(s)
Abe H., Murao K., Imachi H., Cao W.M., Yu X., Yoshida K., Wong N.C., Shupnik M.A., Haefliger J.A., Waeber G., Ishida T.
ISSN
0013-7227
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
145
Numéro
12
Pages
5623-8
Langue
anglais
Notes
Publication types: Journal Article - Publication Status: ppublish
Résumé
Islet-brain-1 (IB1)/c-Jun N-terminal kinase interacting protein 1 (JIP-1) is a scaffold protein that is expressed at high levels in neurons and the endocrine pancreas. IB1/JIP-1 interacts with the c-Jun N-terminal kinase and mediates the specific physiological stimuli (such as cytokines). However, the potential role of the protein in the pituitary has not been evaluated. Herein, we examined expression of the gene encoding IB1/JIP-1 and its translated product in the anterior pituitary gland and a pituitary cell line, GH3. We then examined the potential role of IB1/JIP-1 in controlling TSH-beta gene expression. Exposure of GH3 cells to TRH stimulated the expression of IB1/JIP-1 protein levels, mRNA, and transcription of the promoter. The increase of IB1/JIP-1 content by transient transfection study of a vector encoding IB1/JIP-1 or by the stimulation of TRH stimulates TSH-beta promoter activity. This effect is not found in the presence of a mutated nonfunctional (IB1S59N) IB1/JIP-1 protein. Together, these facts point to a central role of the IB1/JIP-1 protein in the control of TRH-mediated TSH-beta stimulation.
Mots-clé
Adaptor Proteins, Signal Transducing, Animals, Cell Line, Transformed, Gene Expression Regulation, Mutagenesis, Pituitary Gland, Anterior, Rats, Thyrotropin, beta Subunit, Thyrotropin-Releasing Hormone, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 14:48
Dernière modification de la notice
20/08/2019 15:07
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