Primary structure and functional expression of the mouse and frog alpha-subunit of the gastric H(+)-K(+)-ATPase

Détails

ID Serval
serval:BIB_51A4A9F43842
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Primary structure and functional expression of the mouse and frog alpha-subunit of the gastric H(+)-K(+)-ATPase
Périodique
American Journal of Physiology
Auteur(s)
Mathews  P. M., Claeys  D., Jaisser  F., Geering  K., Horisberger  J. D., Kraehenbuhl  J. P., Rossier  B. C.
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
05/1995
Volume
268
Numéro
5 Pt 1
Pages
C1207-14
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
The H(+)-K(+)-ATPase of the gastric parietal cells is responsible for the acidification of the stomach lumen. This heterodimeric protein belongs to the family of cation-translocating P-type ATPases, which includes the closely related Na(+)-ATPase. We have cloned the alpha-subunit cDNA of the Xenopus and murine gastric H(+)-K(+)-ATPase (alpha H-K). We have expressed Xenopus and murine alpha H-K along with the previously cloned gastric H(+)-K(+)-ATPase beta-subunit of rabbit (beta H-K) in Xenopus oocytes by cRNA injection. An antibody directed against the beta H-K coimmunoprecipitates under nondenaturing conditions the alpha H-K of both species, demonstrating assembly of the alpha/beta complex. Additionally, we demonstrate the presence of K(+)-transporting H(+)-K(+)-ATPase in the plasma membrane of oocytes by 86Rb- uptake. The H(+)-K(+)-ATPase-mediated K+ uptake was inhibited by the gastric H(+)-K(+)-ATPase inhibitor Sch-28080, but not by ouabain, and shows K(+)-dependent activation (K1/2 approximately 2 mM). Furthermore, H(+)-K(+)-ATPase-expressing oocytes show a Sch-28080 inhibitable proton extrusion. Our data indicate that the expressed H(+)-K(+)-ATPase behaves functionally in oocytes as in the gastric gland.
Mots-clé
Animals Base Sequence Biological Transport DNA, Complementary/genetics H(+)-K(+)-Exchanging ATPase/*genetics/*metabolism Mice/*metabolism Mice, Inbred BALB C Molecular Sequence Data Oligonucleotide Probes/genetics Oocytes/metabolism Protons Rubidium/metabolism Stomach/*enzymology Tissue Distribution Xenopus laevis/*metabolism
Pubmed
Création de la notice
24/01/2008 13:28
Dernière modification de la notice
03/03/2018 17:11
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