Article: article d'un périodique ou d'un magazine.
Ion transport, ciliary activity, and mechanosensitivity of sinusal mucosa: an in vitro study.
American Journal of Physiology
Date de publication
Publication types: Journal Article
The hypothesis that relative movement between respiratory epithelium and surrounding fluid modulates both ciliary activity and mucosal secretion-absorption properties was tested. Fresh human (HM) and bovine sinusal mucosae (BM) were mounted in transparent chambers perfused with defined media at 36 degrees C. The fluid advanced along the ciliated surface at 15-30 microns/s. Transepithelial transport of electrolytes was studied by using voltage-clamp technique. Ciliary beating frequency (CBF) was recorded by using laser reflectometry. Average transmucosal potential difference (apical side negative) and short-circuit current (Isc) were -0.5 mV and 17 microA.cm-2 for HM and -1.4 mV and 28 microA.cm-2 for BM. Average CBF was 900 beats/min. Ionic pathways included basolateral Na,K-ATPase, K+ channels and symports for Na-Cl and Na-glucose, and apical channels for Na+ (absorption) and for Cl- and K+ (secretion). Increase of fluid velocity up to 300 microns/s induced significant increase of both Isc (63%) and CBF (29%). Such adaptations of transepithelial fluid transport and ciliary activity to hydrodynamic conditions might reflect a mechanism of coordination between the secretion of mucus electrolytes and mucociliary clearance.
Animals, Cattle, Cells, Cultured, Cilia/physiology, Humans, Ion Transport, Mucociliary Clearance, Nasal Mucosa/cytology, Nasal Mucosa/physiology, Stress, Mechanical
Web of science
Création de la notice
Dernière modification de la notice